Rest or REST may refer to:
- To relax or take time off; see leisure or nap
- Rest (album), a 2008 album by Gregor Samsa
- Rest (music), a pause in a piece of music
- Rest (physics), the relation between two observers
- RE1-silencing transcription factor, a human gene
- Representational State Transfer (REST or RESTful), a style of software architecture for distributed hypermedia systems
- Rest (comic), a comic book produced by Milo Ventimiglia, Divide Pictures and Devil's Due Publishing
- Restricted Environmental Stimulation Technique, a sensory deprivation technique
- reStructuredText, a lightweight markup language
- In chemistry, a rest (abbreviated R) is a variable part of a molecule attached to a given core structure; see functional group and side chain
- In cue sports, "rest" is a chiefly British English term for any of a set of mechanical bridges; see Glossary of cue sports terms entry
- In jousting, a rest (from "arrest" meaning to stop) is a cup near the armpit on the side of medieval jousting and cavalry armour
- Bank reserves in the terminology of the Bank of England
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